Analysis of Raffinose Synthase Gene Family in Bread Wheat and Identification of Drought Resistance and Salt Tolerance Function of <i>TaRS15-3B</i>.

At the same time, we explored the physiological mechanisms of the wild-type and overexpression TaRS15-3B lines before and after drought stress ( Figure 17 c–g), and found that after drought stress, compared with the wild-type, the MDA content of the overexpression TaRS15-3B lines showed a highly significant decrease, while the proline content, POD activity, and CAT activity all showed a highly significant increase.

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Under drought stress, the expression levels of TaRS3-1A , TaRS10-2A , TaRS15-3B , and TaRS18-3D all showed a trend of up-regulation followed by down-regulation, but TaRS18-3D was less obviously up-regulated than the other three genes and peaked at 12 h, while the other three genes reached their highest values at 24 h.

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