Leveraging Steric Moieties for Kinetic Control of DNA Strand Displacement Reactions.

Specifically, two categories—unconstrained and constrained—of multi-arm junction complexes were studied. Unconstrained external toehold complexes were found to be capable of decreasing the reaction rate constant by nearly 2 orders of magnitude, while C.C.s were found to be capable of decreasing the reaction rate constant by over 3 orders of magnitude from the control reporter complex. For both categories of U.C.s and C.C.s, a clear trend of decreasing reaction rate constants shows that the more obstructions are added, the smaller the reaction rate constant becomes.

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