Natural variation in codon bias and mRNA folding strength interact synergistically to modify protein expression in Saccharomyces cerevisiae.

Using this approach, we found that the positive association between CAI and logPPR was highly significant ( G = 72.977, df = 1, P = 1.31e-17).

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The tAI term is not significant, while the ensemble Δ G term is weakly significant and has a positive slope, consistent with mF having a weak negative effect on protein accumulation, separate from its positive effects through the interaction.

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