Quantitative SARS-CoV-2 subgenomic RNA as a surrogate marker for viral infectivity: Comparison between culture isolation and direct sgRNA quantification.

Of note, a clear trend of higher sgRNA values was found for +/+ samples respect to -/+ samples (sgRNA E, median [IQR] copies/mL: 140,700 [350–1,071,000] vs and 406 [210–770], p-value = 0.083; SgRNA N, median [IQR] copies/mL: 34,069 [325–306,600] vs 770 [630–980] copies/mL, p-value = 0.165) ( Fig 2 , Panel A and B).

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