Triple Reporter Assay: A Non-Overlapping Luciferase Assay for the Measurement of Complex Macromolecular Regulation in Cancer Cells Using a New Mushroom Luciferase-Luciferin Pair.

NF-κB (RelA) expression showed a trend of increment with increasing treatment TNFα time ( Figure 3 g).

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So, in the A2780 cells with p53-NLuc over-expression (A2780PNLuc) when treated with doxorubicin, a highly significant increase in NLuc activity was observed as expected compared to untreated cells ( Supplementary Figure S6 ). 3.4.

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