Actinobacteria of A2 showed a decreasing trend, corresponding to a sharp increase in its TN and NO 3 − -N content.
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The sentences
Actinobacteria of A3 and A4 showed an increasing trend, probably due to their adaptation and resistance to enzymatic agents.
The Chao1 index also showed a significant trend, indicating that the abundance of microbial communities in A2 and A3 was higher than that in A1, and the abundance of A4 was lower than that in A1.