Transcription factor UBF depletion in mouse cells results in downregulation of both downstream and upstream elements of the rRNA transcription network.

A p value<0.05 was regarded statistically significant (∗), <0.01 very significant (∗∗), <0.001 highly significant (∗∗∗), and <0.0001 extremely significant (∗∗∗∗).

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In all instances, quantification showed a clear trend of increased gene expression of each of these factors, as a function of serum concentration in both WT and shUBF72 cells, and all showed a large decrease of expression in shUBF72, relative to WT, at each serum concentration ( Fig. 9 , A – H ).

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