Therefore, based on the evidence given by an increasing amount of research, control strategies show a clear trend towards molecular techniques, such as polymerase chain reaction (PCR), multiplex PCR, real-time PCR (qPCR), reverse transcriptase PCR (RT-PCR), DNA microarrays, nucleic acid sequence-based amplification (NASBA), isothermal DNA amplification techniques, and next-generation sequencing (NGS) [ 2 ].
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