coli and PMA, contribution of the CD49d- PMNs compartment showed a trend for greater input towards NET formation between 35-40°C. 3.5 CD16+ CD49d+ PMNs have higher FPR1 expression than CD16+ CD49d- PMNs and temperature modulates FPR1 expression in both subsets differently To further examine the phenotype of CD16+ CD49d-/+PMNs, we have also analyzed relative expression of FPR1 that is implicated in pathogen recognition during infection.
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Neutrophil extracellular traps formation and clearance is enhanced in fever and attenuated in hypothermia.
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coli , NETs formed at 35°C had ~14.5% of large fragments when compared to ~6.5% at 37°C (p < 0.05 for both) and while we detected a decreasing trend in large NETs content induced by both stimuli at 40°C, the difference was not statistically significant.