On the other hand, when we inspected the expression of all three virulence genes during 42 hours in the batch culture setup, we observed a reduction in the expression of tcdA, but we did not see a clear trend of downregulation of these toxin genes ( Fig. 2E ).
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<i>Clostridium scindens</i> secretome suppresses virulence gene expression of <i>Clostridioides difficile</i> in a bile acid-independent manner.
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