Returning ryegrass to continuous cropping soil improves soil nutrients and soil microbiome, producing good-quality flue-cured tobacco.

Rhizosphere soil pH showed strong positive correlations with the rhizosphere soil Firmicutes and Bacteroidota and highly significant negative correlations with the rhizosphere soil Proteobacteria, Actinobacteriota, and Fibrobacterota.

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The AN content of bulk soil was relatively high at 45 days after transplanting in 2020 and 2021, which showed a decreasing trend with the extension of the growth period.

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The topological properties of the soil fungal co-occurrence network community after ryegrass rolling showed a trend opposite to that of the soil bacterial community.

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