Screening and Validation of Appropriate Reference Genes for Real-Time Quantitative PCR under PEG, NaCl and ZnSO₄ Treatments in <i>Broussonetia papyrifera</i>.

However, when the least stable gene GAPDH was used for calculation, the expression levels of the DREB and POD showed an overall trend of first increasing, then decreasing, followed by increasing.

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Under the drought stress, when the most stable internal reference genes rRNA , Actin, and their combination ( rRNA + Actin ) were used, the expression levels of the DREB and POD generally showed a trend of increasing first and then decreasing.

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