Optimization of high-throughput 16S rRNA gene amplicon sequencing: an assessment of PCR pooling, mastermix use and contamination.

1 ), the difference in read counts from samples with manually prepared mastermix (median=131658) or premixed mastermix (median=111890) by Mann–Whitney U test comparison did not reach statistical significance ( P =0.05) (Figs S13 and S14).

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