Further study on particle size, stability, and complexation of silver nanoparticles under the composite effect of bovine serum protein and humic acid.

26 With the increase in the concentration of BSA solution, the absorption peaks of AgNPs showed a red shift followed by a blue shift, a phenomenon consistent with the UV results of Mbeta, 27 indicating that the interaction between AgNPs and BSA both occurred and the formed substances existed in solution as AgNPs–BSA complexes; 28 moreover, the absorption intensity of AgNPs with the increase in the BSA concentration showed a trend of increasing and then decreasing, and the color-enhancing effect of the absorption peak could be due to the addition of a low concentration of BSA molecules, which induced Ag + to form AgNPs with a smaller particle size. 29 The main reason for the color reduction effect of the plasmon resonance absorption peak of AgNPs may be that the addition of a high concentration of BSA replaces the sodium citrate molecules on the surface of AgNPs to some extent and mosaics them on the surface of AgNPs in the form of inclusions, forming AgNP–BSA complexes and making the AgNP content in solution lower.

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The peaks at both places show an increasing trend to produce color enhancement.

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