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A miniaturized mode-of-action profiling platform enables high throughput characterization of the molecular and cellular dynamics of EZH2 inhibition.

Sci Rep · 2024 · PMC10799085 · PMID 38242973

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The traces obtained from these experiments contained sufficient kinetic information to determine the rate constants of the fluorescent probe at each temperature tested: as expected, the rates of probe’s association and dissociation kinetics were temperature-dependent, with a clear trend towards faster binding with increasing temperatures (Supplementary Table S1 ).

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