Addition of Vitamin C Mitigates the Loss of Antioxidant Capacity, Vitality and DNA Integrity in Cryopreserved Human Semen Samples.

Consistent with the increase in ROS generation observed following cryopreservation, this process was associated with highly significant overall changes ( p < 0.001) in the lipid peroxidation status of the spermatozoa, as reflected in the percentage of cells positive for the lipid aldehyde MDA ( Figure 4 A).

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An improvement in sperm motility did not reach statistical significance, possibly because additional pathophysiological mechanisms limit the potential effectiveness of antioxidants in rescuing this aspect of sperm function.

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