Effect of very long-term storage and multiple freeze and thaw cycles on 11-dehydro-thromboxane-B<sub>2</sub> and 8-iso-prostaglandin F<sub>2α,</sub> levels in human urine samples by validated enzyme immunoassays.

4 b), while 8-iso-PGF 2α in chromatographic frozen extracts ( n = 212) showed a significant trend toward a decrease starting approximately from month 4 of storage (Fig. 4 c), even though the values of the 2 measurements were still significantly correlated (rho = 0.85, P < 0.0001, n = 212, Fig. 4 d).

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