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Thiolutin has complex effects in vivo but is a direct inhibitor of RNA polymerase II in vitro.

Nucleic Acids Res · 2024 · PMC10954460 · PMID 38214235

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a clear trendno p-value reported
Our experiments showed good reproducibility across replicates ( Supplementary Figures S12A,B ) with a clear trend of increased effects over time of thiolutin inhibition.

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