This conclusion was validated by normalization to the body weight: at E13.5 the size of Igf1r -/- mammary anlage did not differ from that of the wild type embryos (p = 0.11), at E16.5 the sprouts were smaller in the mutants, though the difference did not reach statistical significance (p = 0.08), while at E18.5, the Igf1r -/- mammary glands were significantly smaller (p = 0.000021).
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Mesenchyme instructs growth while epithelium directs branching in the mouse mammary gland.
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