phaffii The preliminary testing of the CRISPR-MAD7 system indicated that the PAM site used for gRNA design might affect the target disruption efficiency of the MAD7 endonuclease but did not show a clear trend ( Table 3 ).
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Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions in <i>Komagataella phaffii</i>.
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