Development of a customizable mouse backbone spectral flow cytometry panel to delineate immune cell populations in normal and tumor tissues.

Furthermore, PDAC samples contained higher levels of PDPN + but a decreasing trend in the percentage of CD31 + cells, although the latter was not statistically significant ( Figure 7C ).

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However, the percentage of B cells showed a trend towards a reduction in Tet2 KO mice ( Figure 8B ), suggesting there are pleiotropic effects of Tet2 loss in the lymphoid lineage.

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We also found that CD8 + T cells showed an increasing trend in PDAC, although two-thirds of these expressed the exhaustion marker PD-1 ( Figure 7C ).

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