Recombinase polymerase amplification combined with lateral flow dipstick assay for rapid visual detection of <i>A.simplex (s. s.)</i> and <i>A.pegreffii</i> in sea foods.

In the future, with the high-throughput detection technology demand, this assay can be improved through the combination of multiple LFD or multiple RPA-LFD assays [ 51 ] because this is an important trend and direction in the field of detection technology. 5 Conclusion In this study, an infect rate investigation and molecular identification of Anisakis in small yellow croaker and mackerel in the East China Sea was conducted, and a visual detection assay was developed based on RPA-LFD.

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