RNA three-dimensional structure drives the sequence organization of potato spindle tuber viroid quasispecies.

Compared to LM samples, a decreasing trend of normalized Shannon entropy was observed in Sys samples, although differences did not reach statistical significance ( Fig 2A and S1 Table ).

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- p17 "Our study also provided novel insights into the constraints on sequence diversity within the quasispecies of viruses" - p3 "Gaining insights into viroid quasispecies evolution is highly significant as it improves our understanding of viral evolution and quasispecies structure." - p2 "These findings emphasize the potential of targeting RNA 3D structure as a more robust approach for antiviral strategies." Reviewer #3: Minor issues: -Lack of information in material and methods about the analysis of the sequencing data.

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