Vertical differentiation drives the changes in the main microflora and metabolites of carbon and nitrogen cycling in the early freeze-thaw period in the Qinghai Lake Basin.

Alpha diversity analysis of soil microorganisms at different elevations in the early freeze–thaw period of the Qinghai Lake watershed ( Figure 2B ) showed that the species richness indices of microorganisms (ACE, Chao1, observed) showed an increasing trend with the increase in elevation.

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The relative abundance of Lactobacillus in group TJ was significantly higher than that in other elevation gradients, and the relative abundance of Azospirillum showed a trend of first increasing and then decreasing with increasing elevation. 3.3.

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