A clear trend was observed in the conversion of 10 mM 1b, with highest product formation obtained with large excess of bicarbonate (55 mM), going in line with observations made with PjCapAB (see ESI, Table S2 † ). 19 The ATP concentration was found to correlate well with the amount of product formed, and in general, some uncoupling can be anticipated, as the amount of formed 2b was found inferior to the ATP concentration used in all cases (see Table 4 ), and a slight excess of ATP was required to reach the highest conversion ( vide infra ).
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Mild hydrolysis of chemically stable valerolactams by a biocatalytic ATP-dependent system fueled by metaphosphate.
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