Rapid sequential clustering of NMDARs, CaMKII, and AMPARs upon activation of NMDARs at developing synapses.

Glutamate-induced GluN1 clusters were largely maintained over time; it appears that there might be a modest decline in the number of clusters seen after a 1 and a 3 day chase versus the earlier time points but this decline did not reach statistical significance ( Figure 3P , filled red circles and filled blue triangles).

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There was a slight increase in NMDAR mEPSCs amplitude under control conditions between 11 and 14 DIV ( Figure 4 ; compare 3 day chase in D with 1 h chase in A) but the increase following NMDAR activation remained statistically highly significant.

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