Optimization of hydrolysis conditions of alginate based on high performance liquid chromatography.

However, with the increase of hydrolysis time, the recovery of alginate hydrolyzed in sulfuric acid and the concentrations of M and G tended to be stabilized, while a decreasing trend was observed in TFA ( Fig. 3 A(a) and 3B(a)).

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The hydrolysis temperature has a greater effect on the degree of hydrolysis, and mass concentration of M and G in the hydrolysate showed a trend of increasing and then decreasing with the increase of temperature, and the content of M and G reached the maximum value at 110 °C ( Fig. 3 C(c)).

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