Combining toll-like receptor agonists with immune checkpoint blockade affects antitumor vaccine efficacy.

While PD-1 expression was not evaluated in those studies, endogenous Tregs in MyC-CaP tumors showed a trend of lower numbers of Treg, but no differences in PD-1 expression on these Treg, following treatment with TLR agonists ( online supplemental figure 10E ).

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