Identification and characterization of the CRK gene family in the wheat genome and analysis of their expression profile in response to high temperature-induced male sterility.

Furthermore, qRT-PCR validation of the six TaCRK genes showed that they play key roles in the development of the mononuclear stage anthers, as all six genes were expressed at highly significant levels in heat-stressed male sterile mononuclear stage anthers compared to normal anthers.

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However, at the trinucleate stage, they showed a decreasing trend but did not reach a significant level.

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In the results of our qRT-PCR experiments, the mononuclear stage of high-temperature sterile anthers showed a trend of higher expression of the TaCRK gene than that of normal anthers regardless of whether it was highly or lowly expressed in the spikelet ( Figs. 11A – 11L ).

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