The Wnt-dependent master regulator NKX1-2 controls mouse pre-implantation development.

Further, using quantitative reverse-transcription PCR (RT-qPCR) to analyze individual embryos, we found that Nkx1-2 KD embryos had a significantly reduced expression of the pre-ribosomal RNA gene 45S and showed a trend for reduced expression of the 18S and 28S rRNAs ( Figure 3 D), further suggesting rRNA transcription impairment.

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Moreover, we observed that Nkx1-2 KD embryos showed highly significant blastomere number reduction and chromosome segregation defects ( Figures 4 A–4I), both of which impair embryo size and development.

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