Effects of chemical <i>in vitro</i> activation versus fragmentation on human ovarian tissue and follicle growth in culture.

5A ), a highly significant upregulation of ENO1 , LDHA , PKM , HIF1A , LIF , and MIF was observed already on Day 1 of culture ( Fig. 5B ).

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In addition, median concentrations of androstenedione, testosterone, and estradiol tended to be lower in the Frag+cIVA group compared to Frag; however, these did not reach statistical significance ( Fig. 3D , Supplementary Table S9 ).

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In general, there was a decreasing trend in most cytokines and chemokines measured across the culture period ( Fig. 4B ).

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