Exploring the in vitro stability of insulin degrading enzyme as a potential biomarker for neurocognitive disorders and Alzheimer's disease risk.

At ten freeze-thaw cycles, the difference became significant—even with a storage duration of 6 months, a difference was observed between samples stored at −20 °C and −80 °C, which, although it did not reach statistical significance, approached a level of significance.

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In addition, there was a difference between samples stored for 6 months and samples thawed three times, but it was only borderline significant ( Table 1 ). 4.4 Storage time No statistically significant differences were found when analyzing samples stored at 4 °C compared to samples stored frozen for 24 h or six months.

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