Multimodal illumination platform for 3D single-molecule super-resolution imaging throughout mammalian cells.

For the comparison between Gaussian and FF epi- and TIRF illumination, representative line scans show a clear trend in signal-to-background ratio, where Gaussian epi-illumination results in the lowest ratio and FF TIRF results in the highest – an improvement of up to 4X compared to Gaussian epi-illumination ( Fig. 2(c) ).

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