Trauma in Neonatal Acute Brain Slices Alters Calcium and Network Dynamics and Causes Calpain-Mediated Cell Death.

When averaged from individual slices, the decay constants increased with longer incubation times for PI + cells and GCaMP-filled neurons but did not reach statistical significance for the latter ( Fig. 3 E ).

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G) Although we found a modestly significant interaction between control and staurosporine-treated slices, post-hoc multiple comparisons confirmed no significant increase in GCaMP6-filled neurons due to staurosporine treatment (Two-way ANOVA, interactions: F(9, 90) = 2.18, p < 0.034, Treatment: F(1, 10) = 0.041, p = 0.93, Sidak’s test for multiple comparisons p > 0.05). aCSF: 5 slices; staurosporine: 8 slices.

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