ASA concentrations > 1000 μM (10,000 μM and 16,000 μM) impaired the mitochondrial metabolic activity, which is equivalent to aerobic cellular respiration, revealing highly significant differences in comparison to the remaining concentrations after 3 (16,000 μM: p < 0.0001–0.01) and 7 (10,000 μM and 16,000 μM: p < 0.0001) days of cell culture with a visible decrease in their respective RFUs.
Additionally, Vukovic et al., who evaluated the effect of lower-dose ASA (10, 50, and 100 μM) on the viability of human DPSCs, did not find a significant difference between the cell viability (MTT-, Neutral Red assay) in the 100 μM of ASA and the control (0 μM) group after 7 days, although a decreasing trend was observed [ 14 ].
However, the photometric quantification of those results showed a strong trend but no significance in comparison to the induction group without ASA.