Additionally, when 10 ng/ml Sema3A was added continuously every two d for 7 d, an increasing trend in ACAN gene expression was confirmed (Fig. 3 C ).
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A p-value < 0.01 was regarded as indicative of a highly significant difference.
Sema3A expression showed a trend consistent with that of COL2A1 , which significantly increased from day 7 after the onset of differentiation and reached a maximum on day 14.