Characterization of human aquaporin ion channels in a yeast expression system as a tool for novel ion channel discovery.

Acetazolamide, a non-specific agent suggested to block AQP water channels, showed a trend towards growth reduction that was not dose-dependent, and might reflect an off-target effect on a sensitive protein such as carbonic anhydrase [ 51 ].

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To test for potentiation of growth, hAQP1-expressing TK2463 cells were treated with the agonist CPT-cGMP; a nonsignificant trend toward increased growth as compared with hAQP1-expressing cells without cGMP was observed (Supp.

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