Feeder-free culture of human pluripotent stem cells drives MDM4-mediated gain of chromosome 1q.

Although abnormal cells were seen at a range of passages, a comparison of cultures sampled at different passage numbers showed a clear trend in the proportion of abnormal karyotypes increasing with the increase in passage numbers ( Figure S3 A), thus reinforcing the importance of minimizing the extent of hPSC expansion in culture ( International Stem Cell et al., 2011 ).

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Remarkably, we found a disparity in the representation of different variants over the years ( Figures 2 A and 2B), with an overall trend toward increase in frequency of aberrations such as gains of chromosome 1q, 20q, and isochromosome 20q at the expense of aberrations such as trisomy 12 and 17 ( Figures 2 A–2D).

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