Single-Molecule Fingerprinting Reveals Different Growth Mechanisms in Seed Amplification Assays for Different Polymorphs of α-Synuclein Fibrils.

4 In Figure 7 H, the overall data for ribbons and fibrils, in low and neutral pH, show a clear trend of faster reactions with higher smSAA diffusion times, reinforcing the idea that secondary nucleation plays a major role in the apparent reactivity in RT-QuiC. αSyn Polymorphs Maintained Their Properties upon Amplification Previous results by Bousset et al. 23 demonstrated that the low-salt ribbons and high-salt fibrils gave rise to different phenotypes when seeded in different buffers, pointing to a loss of properties upon amplification.

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