A 3D Epithelial-Mesenchymal Co-Culture Model of the Airway Wall Using Native Lung Extracellular Matrix.

After ALI culture, the COPD and control hydrogels showed a different trend, where in the control the average length, branchpoints, and endpoint showed a decreasing trend, whereas COPD showed a positive trend for the same metrics.

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After ALI culture, there was a positive trend towards an increase in the number of tubulin-positive cells, Alcian blue-positive cells and MUC5AC-positive cells compared to the submerged cultured epithelial cells ( Figure 8 C).

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ALI-cultured epithelial cells on the collagen I–fibronectin–BSA coating showed a negative trend in KRT5-positive cell proportions compared to submerged culture, which could mean more differentiation at the expense of basal cells.

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The ALI-cultured epithelial cells showed an increasing trend in numbers of all differentiated cell types but no difference in KRT5-positive basal cell proportions ( Figure 8 E). 3.6.

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