Fertility loss and recovery dynamics after repeated heat stress across life stages in male <i>Drosophila melanogaster</i>: patterns and processes.

Now, the probability of mature sperm being present in the seminal vesicles declined in response to adult heat stress ( n = 336, β = −2.137 ± 0.315, Z = −6.780, p < 0.001), with a negative trend for developmental heat stress ( β = −0.503 ± 0.284, Z = −1.770, p = 0.076) and no effect of thermal tolerance ( β = −0.747 ± 0.582, Z = −1.282, p = 0.200; figure 3 a , electronic supplementary material, table S12).

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In a binomial GLMM across n = 182 males from 10 isolines, the four-way interaction was not statistically significant ( β = 1.937 ± 1.659, Z = 1.168, p = 0.243), but day again contributed to several interactions (though some marginally non-significant; electronic supplementary material, table S19).

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