Identification of <i>SikCDPK</i> family genes to low-temperature by RNA-seq approaches and functional analysis of <i>SikCDPK1</i> in <i>Saussurea involucrata</i> (Kar. & Kir.).

After GA 3 , the expression of SikCDPK1 showed a trend of “slightly increasing-decreasing-increasing” ( Figure 6 ), reaching the peak at 6 h, and the expression at the bottom of the peak was 0.816 times of CK, and then continued to increase.

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The expression of snow lotus SikCDPK1 gene in different tissues and organs was calculated using the 2 -ΔΔCt method ( Livak and Schmittgen, 2001 ), and the experimental data were subjected to LSD (least significance difference) multiple ANOVA using the SPSS 18.0 software, with the test of significance set at P <0.05 (significant level) and P <0.01 (highly significant level), and graphs were made in using Excel software. 2.7.

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The relative electricity conductivity and MDA activity of wild type and transgenic tobacco were measured at different treatment times under low temperature stress, and it was found that the relative electricity conductivity of both wild type and transgenic tobacco showed an obvious increasing trend with the extension of the stress time, and the relative conductivity of transgenic tobacco began to decrease after 1 day of recovery at room temperature, while the wild type still maintained an increasing trend; the relative electricity conductivity of transgenic plants was significantly lower than that of wild type during the whole process.

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