did not reach statistical significancep = 0.07765
Interestingly, the percentage of ZsGreen1+cells that co-express FOS was significantly increased in the alcohol group (Mann Whitney test, U = 2, p = 0.0087) while, considering male and female mice separately, the results showed a treatment effect (two-way ANOVA, F 1,8 = 12.64, p = 0.0075) while the effect of sex did not reach statistical significance (two-way ANOVA, F 1,8 = 0.08622, p = 0.07765).