Alfalfa leaf weevil larvae and adults feeding induces physiological change in defensive enzymes of alfalfa.

As the feeding induction time increased, the POD activity showed a decreasing trend, with the lowest POD activity in alfalfa leaves after 72 h of feeding, which was 41.2%, 35.3%, and 41.0% lower than that of healthy alfalfa.

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As the feeding time increased, the CAT activity of alfalfa leaves in each treatment showed a trend of first decreasing and then increasing.

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The CAT activity of alfalfa leaves after treatment with two pests/plant and four pests/plant showed an overall trend of first increasing and then decreasing, with the highest activity occurring after 36 h of feeding.

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