Nuclear localization of MTHFD2 is required for correct mitosis progression.

To validate this observation, we performed retro-transcription quantitative PCR of 20 chromosomal centromeres, which showed a trend towards upregulation of centromeric expression in the absence of MTHFD2 in both asynchronized cells (Fig. 4d ) and cells synchronized in late G2 (Supplementary Fig. 5m ).

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