Multi-omics-driven discovery of invasive patterns and treatment strategies in CA19-9 positive intrahepatic cholangiocarcinoma.

In epithelial cells, the Epi_SLC2A1 showed an increasing trend across both Lineage1 and Lineage2 branches (Supplementary Fig. 8D).

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In trajectory plots, we distinctly tracked a decreasing trend in OXPHOS pathway scores and an increasing trend in glycolytic pathway scores along the trajectories, exhibiting a markedly opposite trend (Fig. 6 A–C).

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Notably, samples with higher hypoxia scores were more common in the CA19-9 positive group, although this difference did not reach statistical significance due to the limited sample size (Supplementary Fig. 8H).

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