4,38,44 All NLP groups showed a trend in zeta potential toward −20 mV, confirming the presence of a protein corona on their surface.
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An investigation of the effect of the protein corona on the cellular uptake of nanoliposomes under flow conditions using quartz crystal microgravimetry with dissipation.
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Prior to protein corona formation, the NTA indicated an average NLP size of approximately 100 nm with an increasing trend in their size after protein corona formation, suggesting that a protein layer was formed around the particles.
These findings suggest that the cell monolayer is less viscoelastic after the attachment of protein corona NLPs, which caused a decreasing trend in the dissipation shift, and that the protein coronation of negatively charged NLPs had the least effect on the cell membrane viscoelasticity.