4 a) showed an increasing trend from G5 to G30 indicating the ability of the nuclei and cells to stretch and fit in 2.5D microgrooves as small as 5 μm.
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Mechanical confinement matters: Unveiling the effect of two-photon polymerized 2.5D and 3D microarchitectures on neuronal YAP expression and neurite outgrowth.
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The sentences
There was a clear trend of alignment of the cells to the direction of the major axis of the microgrooves.