Differential regulation of magnesium transporters Slc41, Cnnm and Trpm6-7 in the kidney of salmonids may represent evolutionary adaptations to high salinity environments.

By contrast to slc41a1-1 , slc41a1-2 did not present any significant difference between groups during PST, just a slight trend to higher expression in the smolt group.

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Further, while cnnm2b is upregulated in FW mefugu, cnnm2b1/2 (we could not distinguish between Ss4R paralogues for technical reasons) presents a clear trend to be upregulated in SW.

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In the case of trpm6-2 and trpm7-1 , difference in relative expression arose from a significant decrease in the parr group, while trpm6-2 displayed an increasing trend (Fig. 5 A; j), and expression of trpm7-1 remained stable (Fig. 5 A; l).

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