Out of the combined cued and generalized contextual fear memory effect, the freezing level explicitly induced by the cued fear memory was estimated by calculating the difference between the freezing levels “During cue” and “Before cue.” This “cued memory score” tended to be lower in the astrocytic ChR2 photoactivated mice compared to the foot shock only control mice, although the difference did not reach statistical significance (Figure 5f ).
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